Journal of Biochemicals and Phytomedicine 2958-8561 5 1 2026 05 05 Phytochemical Analysis and Antimicrobial Efficacy of Calotropis procera Stem Bark Extracts and Isolated Compounds Against Clinical Pathogens 27 33 10.34172/jbp.2026.3 EN Omotayo Oladipupo Oyebanjo https://orcid.org/0009-0000-0352-2750 Oluremi Isola Adeniran https://orcid.org/0000-0001-7235-6549 Ayodeji Olakunle Fatokun https://orcid.org/0000-0001-6581-9699 Journal Article 10.34172/jbp.2026.3 2025 05 05 2025 07 09 Introduction: Calotropis procera is a medicinal plant traditionally used for treating various ailments, but the bioactive constituents of its stem bark require further investigation. This study aimed to evaluate the phytochemical composition and antimicrobial potential of C. procera stem bark extracts and to isolate and characterize active compounds against a panel of clinical isolates. Methods: Methanol and ethyl acetate extracts of the stem bark were subjected to phytochemical screening. Their antimicrobial activity was assessed against clinical isolates of methicillin‑resistant Staphylococcus aureus (MRSA), Escherichia coli, Pseudomonas aeruginosa, Candida albicans, and Salmonella typhi using agar well diffusion, broth dilution for minimum inhibitory concentration (MIC), and minimum bactericidal/fungicidal concentration (MBC/MFC) assays. Bioassay‑guided fractionation of the active ethyl acetate extract led to the isolation of three compounds (SCP‑A, SCP‑B, SCP‑C) using column chromatography. Results: Phytochemical analysis confirmed the presence of tannins, saponins, flavonoids, and other secondary metabolites. The methanol extract demonstrated broad‑spectrum activity, particularly against MRSA, E. coli, P. aeruginosa, and C. albicans, with MIC values ranging from 0.63 to 1.25 mg/mL. The isolated compounds SCP‑A and SCP‑B exhibited significant and superior antimicrobial effects compared to the crude extracts, showing potent activity against most tested pathogens. SCP‑C showed more limited activity. Conclusion: The stem bark of C. procera contains bioactive compounds with promising antimicrobial properties against key clinical pathogens, including multidrug‑resistant MRSA. These findings justify further investigation into the development of these compounds as potential antimicrobial agents.